9:00 investigation of the cell wall STRUCTURE simplicity of chlorophyll and extraction in the SUB-AERIAL micro-algae, ** * J. Griner, J. Trull, and YES Nienow, Valdosta State University in Valdosta GA 31698th Subaerial algae are a heterogeneous group of micro-organisms that can survive on land in environments where liquid water is rare. The physiological adaptations, with whom they live in these environments are not clear. Two important factors likely to be encouraged. First, many have subaerial algae cell walls hydrophobic, a fact which seems to contradict around the water quickly. Secondly, the chlorophyll is difficult to extract many subaerial algae with standard methods. We want to know if these two characteristics are correlated simultaneously by different strains of subaerial test for algae and cyanobacteria and Hydro phobia simple extraction of chlorophyll. To determine Hydrophobic, we agree with the standard microbiological compliance hydrocarbons (MATH) method in cells buffer are mixed vigorously in the presence of Hexadecane. Hydrophobic cells, as these droplets Hexadecane and remain at the top of phase. The degree of compliance with photometry. The chlorophyll is extracted with cold acetone (the default method) or hot, followed by DMSO cold acetone. The amount of chlorophyll in the extract is default equations. Our preliminary results indicate that the extract of hot DMSO may contain more than twenty times the amount of chlorophyll subaerial some algae extracts can be cold acetone.

9:15 phip DNA damage induced and the inhibitory effect NAC and GHS. * Ashok Jain, Department of Natural Sciences, Albany State University in Albany, Georgia. Breast cancer is the second leading cause of death of cancer cases among women. There are lots of chemicals from the environment, which estrogenic and are of the opinion that compared with an increased risk of developing breast cancer. The supply of high-industrialized nations like the USA, is strong meat. However, the Asian diet is made up of more fruits, vegetables and soy products. Heterozyklische Amine (HCAs), where the beef products such as meat, chicken, pork and fish are cooked at high temperatures. These carcinogenic amines have been identified as important and are regarded as risk factors for cancer-Initiation. 2-l-amino-6-methyl-phenylimidazol [4, 5-b] pyridine (phip) is the most common HCA found well done and grilled meat, therefore the objective of this study was to analyse the positive impact of antioxidants, fruits and vegetables to prevent the toxic effects of phip. The culture of epithelial cells breast cells (MCF 10A) was launched and phip for 3 hours. After treatment cells were harvested and DNA strands has been quantified failures by measuring the average olive tail movement following each cell gel electrophoresis (test comets). To study a possible role of antioxidants in protecting phip toxicity, the capacity of the three anti-oxidants (ascorbic acid [ASA - 10mm], Glutthione [GHS - 10mm], and N-acetyl-cysteine [NAC - 10mm]) , Were discussed. GHS and NAC show protective effect of cells MCF 10A of the tail olive movement was briefly, indicating GHS and NAC are able to repair the breaks strands of DNA. On the other hand, ASA is not a protective effect of cells MCF 10A. Other studies in this area, identify interactions genotoxicity / heterozyklische amines and carcinogenic effects of chemopreventive to food supplements

9:00 CELL WALL AND INVESTIGATION OF THE STRUCTURE OF EASE CHLOROPHYLLE EXTRACTION AND SUB-MICRO-ALGEN AERIAL ** * Griner J., J. Trull, and YES Nienow, Valdosta State University, Valdosta GA 31698th Subaerial algae are a heterogeneous group of micro-organisms can survive on land in environments where water is scarce. The physiological adaptations that permit, they live in these environments are not clear. Two factors may be important, were encouraged. First, a lot of subaerial hydrophobic algae cell walls, a fact that is clearly in contradiction with, around water quickly. Secondly, the chlorophyll is difficult to extract many subaerial algae with standard methods. We want to know whether these two characteristics are correlated simultaneously by different strains tested by subaerial algae and cyanobacteria of the hydrophobicity and the ease of extracting chlorophyll. To determine hydrophobicity, we agree with the standard micro-compliance hydrocarbons (MATH), a method in which the cells are exposed in buffer mix vigorously in the presence of hexadécane. Hydrophobic cells to hexadécane droplets and remain in the upper stage. The degree of respect for photometry. Chlorophyll is with cold acetone (the default method) or DMSO hot, cold, followed by acetone. The amount of chlorophyll in the excerpt is standard equations. Our preliminary results indicate that the extract of hot DMSO may contain more than twenty times the amount of chlorophyll subaerial some seaweed extracts can be of acetone from the cold.

9:15 phip induced DNA damage and the inhibitory effects of NAC and GHS. * Ashok Jain, Department of Natural Sciences, Albany State University in Albany, Georgia. Breast cancer is the second leading cause of cancer deaths among women. There are a lot of chemicals in the environment, estrogen and opinion are connected with an increased risk of breast cancer. Feeding highly industrialized countries like the United States, there is a lot of meat. However, the Asian diet is composed of more fruits, vegetables and soy products. Heterozyklische Amine (HCAs), where the beef products such as meat, chicken, pork and fish are cooked at high temperatures. These amines have been identified as carcinogenic and are regarded as risk factors for cancer initiation. 2-l-amino-6-methyl-fenilimidazol [4, 5-b] pyridine (PhIP), the HCA done well in many grills and therefore the purpose of this study was to understand the positive impact of antioxidants, In fruits and vegetables, in order to avoid the toxic effects of PhIP. The culture of mammary epithelial cells (MCF 10A), and was launched with PhIP for 3 hours. After treatment, cells were harvested and breaks DNA was quantified by measuring the movement mean olive tail following single cell gel electrophoresis (test comets). For the study of a possible role of antioxidants in protecting the PhIP toxicity, the ability of the three antioxidants (ascorbic acid [AoA - 10mM], Glutthione [GHS - 10mM], and N-acetyl cysteine [NAC - 10mM ]), were studied. GHS and NAC show protective effect in cells MCF 10A as the tail olive movement was briefly, indicating GHS and NAC are in a position to cuts DNA repair. On the other hand, not to show AoA protective effect in cells MCF 10A. Other studies in this area, identifying interactions genotoxicity / heterozyklische amines and chemopräventive carcinogenic effect of dietary supplements.

9:30 MYCOPLASMA AND SALMONELLA IN GOPHER TORTOISES MOODY AIR FORCE BASE, GEORGIA, J. Mitchell Lockhart * (1) and W. Gregory Lee (2) (1), Department of Biology, Valdosta State University, Valdosta, Georgia, 31698, and (2) Department of the Air Force. Since 1999 we have a population of gopher tortoises at Moody Air Force Base, and Lowndes counties Lanier, Georgia for the presence of Upper Respiratory Tract Disease. The study, later, study the chemistry connection plasma or protein electrophoresis, analysis of nearly 100 plasma samples for chemistry variables plasma 20-25, kinship population genetics separate. Salmonella and Mycoplasma presence and population base with regard to the military mission. Our results have already been five suspects turtles and positive for mycoplasma, turtles and 3 were positive for salmonella. Recently, we started with a turtle monitoring relatively new remote Frequency Identification of the technique are presented.

9:45 federal restoration threatened endemic GEORGIA BAPTISIA ARACHNIFERA (FABACEAE), in the biological station LAKE LOUISE, LOWNDES COUNTY, GA, John B. Pascarella, Valdosta State University, Valdosta, GA 31698th An experiment ex situ populations of threatened federal endemic Georgia legume arachnifera Baptisia (Fabaceae), there is on Lake Louise (LL) Biological Field Station in Lowndes County, GA. I studied the success of the restoration of B. Arachnifera seeds and improve the plantations and transplantations. While normally the average greenhouse germination 70% of seed germination in the desert to 15.5% (Fall 2004) and 16.5% and 19% in both studies (Spring 2005). The seed germination was the highest in an open society, and not for salaries, followed by burying the waste, no litter treatments. Plants, not 0% for 2005, survival in an area shaded Woody (not since 1998), and 6% for survival in a previously burned (2005). The five months 256 transplanted seedlings in the spring of 2005, 86.2% survival rate for the next few years (2005-2006). Not with flowers and plants, either in 2005 or 2006. Both 49 years, the plants planted in the fall of 2004, 85.7% of the survival during 1 year after transplantation and survival 97.6% in the second year. Flowers percentage rose from 14% in the first year to 38% in the second year. To maximize the number of poor people in time, this study suggests that the collection of seeds from several plants, they grow in a greenhouse, and replanting is <1 year 1-2 years or plants.